ISSN 2630-0583 (Print)

ISSN 2630-0656 (Online)

JCST

Journal of Current Science and Technology

http://jcst.rsu.ac.th

Journal of Current Science and Technology. Vol.9 No.1 , January - June 2019.

Cytotoxicity and induction of the apoptotic activity of hirsutinolide series/sesquiterpene lactones from Vernonia cinerea on human colorectal cancer cells (COLO 205)

Suchada Jongrungruangchok, Nalinee Pradubyat, Thanapat Songsak, Faongchat Jarintanun, Marisa Wall, Leng Chee Chang, and Supakit Wongwiwatthananukit

Abstract

          Hirsutinolide series/sesquiterpene lactones from Vernonia cinerea, were investigated for cytotoxic effects and induction of the apoptotic activity on human colorectal cancer cells (COLO 205).  The results showed that hirsutinolide series significantly inhibited cell viability of COLO 205 with IC50 less than 1.5 μM.  Flow cytometric analysis indicated that hirsutinolide series (compounds 7, 10, 11, and 22) inhibited DNA synthesis as evidenced by a decline of DNA content in S, G2/M, and G0/G1 phases of the cell cycle.  Furthermore, hirsutinolide series provided anticancer activity by inducing apoptosis on COLO 205 cells through the downregulation of anti-apoptotic genes; Bcl-2 and Bcl-xL.

Keywords: COLO 205, cytotoxicity, hirsutinolide series/sesquiterpene lactones, induction of apoptotic activity, Vernonia cinerea

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