ISSN 2630-0583 (Print)

ISSN 2630-0656 (Online)

JCST

Journal of Current Science and Technology

http://jcst.rsu.ac.th

Rangsit Journal of Arts and Sciences. Vol.5 No.2 , July - December 2015.

Prevalence of Pfcrt 76 and Pfmdr1 86 mutations in Plasmodium falciparum isolates from endemic areas of Thailand by multiplex nested PCR-RFLP

Kanyanan Kritsiriwuthinan and Warunee Ngrenngarmlert

Abstract

          Plasmodium falciparum drug resistance is a major factor affecting malaria spread and mortality rates of malaria infections.  Several studies have shown that chloroquine and other antimalarial drug resistant P. falciparum are associated with a mutation of the Pfcrt (K76T) and Pfmdr 1 (N86Y) genes.  The aim of this study is to investigate the prevalence of these two gene mutations in Thailand.  A total of 84 P. falciparum infected blood samples were collected from eight malaria-endemic provinces.  A multiplex-nested PCR-RFLP was used to examine the two gene mutations.  The results revealed a very high prevalence (100%) of Pfcrt (K76T) gene mutation and a high prevalence (52.4%) of Pfmdr1 (N86Y) gene mutation.  These findings suggest that in Thailand P. falciparum is resistant to chloroquine and that there is an increasing trend of other drug-resistant malaria.  The highest and lowest prevalence of these double genes mutations were found in Mae Hong Son province (100%) and Trang province (25%).  This study is useful for the epidemiology of P. falciparum drug resistance, and can support the selection of appropriate antimalarial drug treatment and drug resistance surveillance and control programs.

Keywords: Plasmodium falciparum, Pfmdr1, Pfcrt, prevalence of gene mutations, multiplex-nested PCR-RFLP

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