ISSN 2630-0583 (Print)

ISSN 2630-0656 (Online)


Journal of Current Science and Technology

Rangsit Journal of Arts and Sciences. Vol.4 No.1 , January - June 2014.

In vitro kill-time test of disinfectants against Pseudomonas aeruginosa recovered from water associated with hemodialysis applications

Patamaporn Sukplang and Acharawan Thongmee


             Bacterial contamination causes various problems in patients with kidney disease undergoing hemodialysis.  Pseudomonas aeruginosa is found as a major contaminant in dialysis apparatus, therefore, effective disinfection to prevent the buildup of this bacteria in the system is needed.  The aim of this study was to evaluate the effective concentration and required kill time of disinfectants commonly used for disinfection of dialysis apparatus against Pseudomonas aeruginosa.  Antibacterial activities of five disinfectants, sodium hypochlorite, hydrogen peroxide, formalin, Perxania 2505 (5% peracetic acid) and Benzalkonium chloride were evaluated. They were tested against P. aeruginosa isolated from contaminated dialysis apparatus. The evaluation procedures included minimal bactericidal concentration (MBC) assay and kinetics of killing assessment.  It was shown that Perxania possesses the lowest MBC required for killing P. aeruginosa (0.2 ppm) while the MBCs of sodium hypochlorite, hydrogen peroxide, formalin and Benzalkonium chloride are 200, 2, 370 and 1000 ppm, respectively.  Kinetics of killing of each disinfectant was performed at MBC values of each disinfectant to kill 106 CFU/ml inoculum of tested bacteria.  The results showed that Perxania killed 3 logs (99.9%) of P. aeruginosa in 58 minutes.  In conclusion, the in vitro kinetics of kill studies demonstrate that Perxania 2025 (PAA) rapidly kills P. aeruginosa recovered from water associated with hemodialysis system.  The speed of kill observed for PAA is faster than that for other test disinfectants under the same test conditions.

Keywords: Pseudomonas aeruginosa, hemodialysis, kill-time

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