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Journal of Current Science and Technology

ISSN 2630-0656 (Online)

Polyphenol and antioxidant activities of Kombucha fermented from different teas and fruit juices

  • Vanida Osiripun, Faculty of Food Technology, College of Agricultural Innovation and Food Technology, Rangsit University, Patumthani 12000, Thailand, Corresponding author; E-mail: vanida.o@rsu.ac.th
  • Tarit Apisittiwong, Faculty of Food Technology, College of Agricultural Innovation and Food Technology, Rangsit University, Patumthani 12000, Thailand


There are many inexpensive beverages with beneficial effects to the body such as preventing cancer, inhibiting heart disease, reducing cholesterol, and blood pressure, inhibiting diabetes, and developing the immune.  In this study we assessed the polyphenol and antioxidant activities of Kombucha fermented from three sources: black tea, green tea, and oolong tea due to their high phenolic content and fruit juices such as pineapple, apple and pomegranate juices due to their strong antioxidant activity.  The highest total phenolic content was found in oolong tea Kombucha fermented with pomegranate juice (994.42 mg GAE/L).  While oolong tea Kombucha fermented with apple juice had the highest antioxidant activity (1,273.74±4.15 mg AAE/L).  The main components found in these Kombucha are acetic acid, lactic acid, glucuronic acid, sucrose, fructose, and ethanol.  A panel of 60 participants ranked the juices using a 9 point Hedonic scale, with pomegranate and pineapple juice receiving the highest scores.  This clearly indicated that these Kombucha fermented from black tea, green tea and oolong tea with pineapple juice, apple juice and pomegranate juice not only contributed their enormous polyphenols and antioxidant activities to promote health and well-being, but also improved sensory properties.

Keywords: antioxidant activities, black tea, fruit juice, green tea, Kombucha, polyphenol, oolong tea

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DOI: 10.14456/jcst.2021.20


AOAC. (2005). Official methods of analysis of the Association Analytical Chemists. 18th ed.Gaithersburg.

Akbarirad, H., Mazaheri Assadi, M., Pourahmad, R., & Mousavi Khaneghah, A. (2017). Employing of the different fruit juices substrates in vinegar kombucha preparation. Current Nutrition & Food Science13(4), 303-308. DOI: 10.2174/1573401313666170214165641

Alejandra, V. S., Beaufort, S., Bouajila, J., Souchard, J. P., & Taillandier, P. (2018). Understanding Kombucha tea fermentation: A review. Journal of Food Science, 83(3), 580-588. DOI: https:// doi 10.1111/1750-3841.14068

Alejandra, V. S., Beaufort, S., Bouajila, J., Souchard, J. P., & Taillandier, P. (2019). Impact of fermentation conditions on the production of bioactive compounds with anticancer, anti-inflamatory and antioxidant properties in kombucha tea extracts. Process Biochemistry, 83(3), 44-54. DOI: https:// doi org/10.1016/j.procbio.2019.05.004

Amarasinghe, H., Weerakkody, N. S., & Waisundara, V. Y. (2018). Evaluation of physicochemical properties and antioxidant activities of Kombucha “Tea Fungus” during extended period of fermentation. Food Science Nutrition Journal, 6(3), 659- 665. DOI: https://doi.org/10.1002/fsn3.605

Aviram, M., Volkova, N., Coleman, R., Dreher, M., Reddy, M. K., Ferreira, D., & Rosenblat, M. (2008). Pomegranate phenolics from the peels, arils, and flowers are antiatherogenic: Studies in vivo in atherosclerotic apolipoprotein E-deficient (E-o) mice and in vitro in cultured macrophages and lipoproteins. Journal of Agricultural and Food Chemistry, 56(3), 1148-1157. DOI; 10.1021/jf071811q

Ayed, L., Abid, S. B., & Hamdi, M. (2017). Development of a beverage from red grape juice fermented with the Kombucha consortium. Annual Microbiology, 67(1), 111-121. DOI: 10.1007/ s13213-016-1242-2

Barreira, J. C. M., Arraibi, A. A., Isabel, C. F. R., & Ferreira, I. C. F. R. (2019). Bioactive and functional compounds in apple pomace from juice and cider manufacturing: Potential use in dermal formulations. Trends in Food Science and Technology, 90, 76-87. DOI: https://doi.org/10.1016/j.tifs.2019.05.014

Cvetković, D., Ranitović, A., Savić, D., Joković, N., Vidaković, A., Pezo, L., & Markov, S. (2019). Survival of wild strains of Lactobacilli during kombucha fermentation and their contribution to functional characteristics of beverage. Polish Journal of Food and Nutrition Sciences, 69(4), 407-415. DOI: https://doi: 10.31883/pjfns/112276

Essawet, N. A., Cvetkovic, D., Velicanski, A., Brunet, C., Vulic, J., Maksimovic, V., & Sinisa, M. (2015). Polyphenol and antioxidant activities of Kombucha beverage enriched with coffee berry extract. Chemical Industry and Chemical Engineering Quarterly, 21(3), 399-409. DOI: https:// doi. org/ 10. 2298/ CICEQ140528042E.

Ferreira, E. A., Siqueira, H. E., Vilas Boas, E. V., Hermes, V. S., & Rios, A. O. (2016). Bioactive compounds and antioxidant activity of pineapple fruit of different cultivars. The Revista Brasileira de Fruticultura, 38(3), 1-7. DOI: http://dx.doi.org/10.1590/0100-29452016146

Gamboa-Gomez, C., Gonzalez-Laredo, R. F., Gallegos-Infante, J. A., Perez, M. L., Martha,R., Moreno-Jimenez, M. R. M., Flores-Rueda, A. G., Rocha-Guzman, N. E. (2016). Antioxidant and angiotensin - converting enzyme inhibitory activity of Eucalyptus Camaldulensis and Litsea Glaucescens infusions fermented with Kombucha consortium. Food Technology, 54(3), 367-374. DOI: 10.17113/ft b.

Jafaria, R., Naghavia, N. S., Daranib, K. K., Doudia, M., & Shahanipour, K. (2020). Kombucha microbial starter with enhanced production of antioxidant compounds and invertase. Biocatalysis and Agricultural Biotechnology, 29, 101789. DOI: https://doi.org/10.1016/j.bcab.2020.101789

Jayabalan, R., Malbasa, R. V., Loncar, E. S., Vitas, J. S., & Sathishkumar, M. (2014). A review on kombucha tea microbiology, composition, fermentation, beneficial effects,toxicity, and tea fungus. Comprehensive Reviews in Food Science and Food Safety, 13(4), 538-550. DOI: https://doi.org/10.1111/1541-4337.12073

Jayabalan, R., Malbaša, R. V., Sathishkumar, M. (2015). Kombucha Tea: Metabolites. In: Merillon JM., Ramawat K. (eds) Fungal Metabolites. Reference Series in Phytochemistry. Springer, Cham. DOI: https://doi.org/10.1007/978-3-319-19456-1_12-1

Kappeng, K., & Pathom-aree, W. (2009). Isolation of acetic acid bacteria from honey. Maejo International Journal of Science and Technology, 3(01), 71-76. DOI: https://www.researchgate.net/publication/285968663

Malbasa, R., Vitas, J., Loncar, E., Grahovac, J., & Milanovic, S., (2014). Optimisation of the antioxidant activity of Kombucha fermented milk products. Czech Journal of Food Science, 32(5), 477-484. DOI: https:// doi. org/ 10. 17221/ 447/ 2013-CJFS

Manasathien, J., Indrapichate, K., & Intarapichet, O. K. (2012). Antioxidant activity and bioefficacy of pomegranate Punica granatum Linn. peel and seed extracts. Global Journal of Pharmacology, 6(2), 131-141. DOI: 10.5829/idosi.gjp.2012.6.2.64226

Marques, M. R., Paz, D. D., Batista, L. P. R., Barbosa, C. O., Araújo, M. A. M., & Moreira-Araujo, R. S. R. (2012). An in vitro analysis of the total phenol content, antioxidant power, physical, physicochemical, and chemical composition of Terminalia catappa Linn fruits. Ciência Tecnology Aliment, 32(1), 209-213. DOI: https:// doi. org/ 10. 1590/ S0101 - 20612012005000023

Martínez Leal, J., Valenzuela Suárez, L., Jayabalan, R., Huerta Oros, J., & Escalante-Aburto, A. (2018). A review on health benefits of kombucha nutritional compounds and metabolites. CyTA-Journal of Food, 16(1), 390-399. DOI: https://doi.org/10.1080/19476337.2017.1410499

Naczk, M., & Shahidi, F. (2004). Extractions and analysis of phenolics in food. Journal of Chromatography A, 1054(1-2), 95-111.

Nummer, B. A., (2013). Kombucha brewing under the food and drug administration model food code: risk analysis and processing guidance. Journal Environmental Health, 76, 8-11.

Orgil, O., Schwartz, E., Baruch, L., Matityahu, I., Jamal Mahajna, J., & Amir, R. (2014). The antioxidative and anti-proliferative potential of non-edible organs of the pomegranate fruit and tree. Food Science and Technology, 58, 571-577. DOI: https://doi.org/10.1016/j.jff.2016.08.024

Quideau, S., Deffieux, D., Douat-Casassus, C., & Pouysegu, L. (2011). Plant polyphenols: chemical properties, biological activities, and synthesis. Angewandte Chemie, 50(3), 586-621. DOI: http://dx.doi.org/10.1002/anie.201000044

Ramalho, L. A., & Mascheroni, R. H. (2012). Quality evaluation of pineapple fruit during drying process. Food and Bioproducts Processing, 90, 275-283. DOI: 10.1016/j.fbp.2011.06.001

Ramsaroop, R. E. S. & Saulo, A. A. (2007). Comparative consumer and physicochemical analysis of Del Monte Hawai`i gold and Smooth Cayenne pineapple cultivars. Journal of Food Quality, 30, 135-159. DOI: https://doi.org/10.1111/j.1745-4557.2007.00111.x

Seeram, N. P., Zhang, Y., Reed, J. D., Krueger, C. G., & Vaya, J. (2006). Pomegranate phytochemicals. In N. P. Seeram, R. N. Schulman, & D. Heber (Eds.). Pomegranates ancient roots to modern medicine (pp. 3-29). http://refhub.elsevier.com/S0023-6438(14)00180-7/sref43

Siddiqui, N., Rauf, A., Latif, A., & Mahmood, Z. (2017). Spectrophotometric determination of the total phenolic content, spectral and fluorescence study of the herbal Unani drug Gule-Zoofa (Nepetra bracteata Benth). Journal of Taibah University Medical Sciences, 12(4), 360-363. DOI: 10.1016/j.jtumed.2016.11.006

Tu, S. H., Chen, L. C., & Ho, Y. S. (2017). An apple a day to prevent cancer formation: Reducing cancer risk with flavonoids. Journal of Food and Drug Analysis, 34(1), 29-40. DOI: https://doi.org/10.1016/j.jfda.2016.10.016

Yikmis, S., & Tuggum, S. (2019). Evaluation of microbiological, physicochemical and sensorial properties of purple basil Kombucha beverage. Turkish Journal of Agriculture Food Science and Technology, 7(9), 1321-1327. DOI: https:// doi. 10.24925/turjaf.v7i9.1321-1327.2550

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