The extraction of lipid from microalgae found in brackish water by terpenes
- Sasirindara Labua, Faculty of Food Technology, College of Agricultural Innovation and Food Technology, Rangsit University, Patum Thani, Thailand, Corresponding author; E-mail: email@example.com
- Tanatya Kenkhunthot, Faculty of Agricultural Innovation, College of Agricultural Innovation and Food Technology, Rangsit University, Patum Thani, Thailand
In this research, terpenes were used to evaluate the extraction of lipids from microalgae found in brackish water. p-Cymene, α-pinene, and d-limonene terpenes were used instead of petroleum-based solvents, which have a low boiling point and are toxic as well as highly flammable. The physical and thermal pretreatment method of grinding and then autoclaving at a temperature of 121°C and a pressure of 15 lb/in2 was investigated to enhance the lipid extraction efficiency and excrete the intracellular lipid. The amount of lipid extracted by p-cymene was 29.23 ± 0.25 % (lipid wt./dry.wt. microalgae), whereas the amount of lipid extracted by n-hexane:methanol 3:1( v/v) was 13.5 ± 0.20 % (lipid wt./ dry.wt. microalgae). The quantity of fatty acid extracted from the microalgae was determined using GC-MS analysis. The results showed that the two main fatty acids were palmitic acid (C6H12O2; 16:0) and oleic acid (C18H34O2; C18:1). There was more palmitic acid (16:0) than oleic acid (C18:1) at 7.70% and 7.08%, respectively. This research found that using natural solvents to extract lipid from microalgae could be a feasible and sustainable alternative to petroleum solvents which are toxic, environmentally unfriendly and increase greenhouse gases causing climate change.
Chen, Z., Wang, L., Qiu, S., & Ge, S. (2018). Determination of microalgal lipid content and fatty acid for biofuel production. BioMed Research International. Article ID 1503126, 17 pages, 2018, 1-17. DOI: https://doi.org/10.1155/2018/1503126.
Cho, K. S., Lim, Y., Lee, K., Lee, J., Lee, J. H., & Lee, I. (2017). Terpenes from forests and human health. Toxicologyg Research, 33(2), 97-106. DOI: 10.5487/TR.2017.33.2.097.
Grazhdannikov, A. E., Kornaukhova, L. M., Rodionov, V. I., Pankrushina, N. A., Shults, E. E., Fabiano-Tixier, A. S., . . . Chemat, F. (2018). Selecting a green strategy on extraction of birch bark and isolation of pure botulin using monoterpenes. ACS Sustainable Chemistry & Engineering, 6, 6281-6288. DOI: 10.1021/acssuschemeng.8b00086
Halim, R., Danquah, M. K., & Webley, P. A. (2012). Extraction of oil from microalgae for biodiesel production: A Review. Biotechnology Advances. 30(3), 709-732. DOI: 10.1016/j.biotechadv.2012.01.001
Ivankovic, A., Dronjić, A., Bevanda, A. M., & Talić, F. (2017). Review of 12 principles of green chemistry in practice. International Journal of Sustainable and Green Energy, 6(3), 39-48. DOI: 10.11648/j.ijrse.20170603.12
Kumar, R. R., Rao, P. H., & Arumugam, M. (2015). Lipid extraction methods from microalgae: a comprehensive review. Frontiers in Energy Research, 2, 1-9. DOI: https://doi: 10.3389/fenrg.2014.00061
Labua, S., Inprasit, N., & Ngamcharoen, W. (2015). Scale-up and cultivation of microalgae from brackish water in Thailand in transparent high density polyethylene bags. Rangsit Journal of Arts and Sciences 5(2):78-91. DOI: 10.14456/rjas.2015.15
Li, Y., Naghdi, F. G., Garg, S., Adarme-Vega, T. C., Thurecht, K. J., Ghafor, W. A., . . . Schenk, P. M. (2014). A comparative study: the impact of different lipide methods on current microalgal lipid research. Microbial Cell Factories, 13, Article No. 14, 14-22. DOI: https://doi: 10.1186/1475-2859-13-14.
Madji, S., Hilali, S., Fabiano-Tixier, A. S., Tenon, M., Bily, A., Laguerre, M., & Chemat, F. (2019). para-menthane as a stable terpene derived from orange by-products as a novel solvent for green extraction and solubilization of natural substances. Molecules, 24(11), 2170-2182. DOI: 10.3390/molecules24112170
Mamidipally, P. K., & Liu, S. X. (2004). First approach on rice bran oil extraction using limonene. European Journal of Lipid Science and Technology, 106(2), 122-125. DOI: https://doi:10.1002/ejlt.20030089
Matyash, V., Liebisch, G., Kurzchalia, T. V., Shevchenko, A., & Schwudke, D. (2008). Lipid extraction by methyl-tert-butyl ether for high-throughput lipidomics. Journal of Lipid Research, 49(5), 1137-1146. DOI: 10.1194/jlr.D700041-JLR200
Rashid, U., Anwar, F., Moser, B. R., & Knothe, G. (2008). Moringa oleifera oil: a possible source of biodiesel. Bioresource Technology, 99(17), 8175-8179. DOI: 10.1016/j.biortech.2008.03.066
Schwab, W., Davidovich-Rikanati, R., & Lewinsohn, E. (2008). Biosynthesis of plant-derived flavor compounds. Plant Journal, 54(4), 712-732. DOI: 10.1111/j.1365-313X.2008.03446.x.
Tanzi, D. C., Vian, M. A., Ginies, C., Elmaataoui, M., & Chemat, F. (2012). Terpenes as green solvents for extraction of oil from microalgae. Molecules, 17, 8196-8205. DOI: 10.3390/molecules17078196